Abstract
Riboswitches are composed of two regions: one for binding to the ligand (the aptamer domain) and the other for regulating the expression of the gene (the expression platform). In most riboswitches (both natural and artificial), a part of the aptamer domain required for ligand binding is directly involved in the regulation of expression, so that it is difficult to design other ligand-responsive riboswitches based on these riboswitches even by using artificial aptamers obtained through in vitro selection. This chapter describes a method for rationally constructing a foundational ON-riboswitch, which is easily available for the design of other ligand-dependent riboswitches, by introducing a new region (a modulator sequence: MS) in addition to the two basic regions. A facile method for preparing arbitrary molecule-dependent riboswitches based on the foundational riboswitch is also presented.
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Acknowledgments
This work was supported by ‘Special Coordination Funds for Promoting Science and Technology’ and ‘Grant-in-Aid for Scientific Research on Innovative Areas’ from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
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Ogawa, A. (2014). Rational Design of Artificial ON-Riboswitches. In: Ogawa, A. (eds) Artificial Riboswitches. Methods in Molecular Biology, vol 1111. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-755-6_12
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DOI: https://doi.org/10.1007/978-1-62703-755-6_12
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Publisher Name: Humana Press, Totowa, NJ
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