Abstract
Animal models are valuable tools for investigating the in vivo pathogenesis of Staphylococcus epidermidis infections. Here, we present the procedure for generating a central nervous system catheter-associated infection in a mouse, to model the central nervous system shunt infections that frequently complicate the treatment of hydrocephalus in humans. This model uses stereotactic guidance to place silicone catheters, pre-coated with S. epidermidis, into the lateral ventricles of mice. This results in a catheter-associated infection in the brain, with concomitant illness and inflammation. This animal model is a valuable tool for evaluating the pathogenesis of bacterial infection in the central nervous system, the immune response to these infections and potential treatment options.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
McGirt MJ, Zaas A, Fuchs HE et al (2003) Risk factors for pediatric ventriculoperitoneal shunt infections and predictors of infectious pathogens. Clin Infect Dis 36:858–62
Kockro RA, Hampl JA, Jansen B et al (2000) Use of scanning electron microscopy to investigate the prophylactic efficacy of rifampin-impregnated CSF shunt catheters. J Med Microbiol 49:441–450
Fux CA, Quigley M, Worel AM et al (2006) Biofilm-related infections of cerebrospinal fluid shunts. Clin Microbiol Infect 12:331–337
Snowden J, Beaver M, Smeltzer M et al (2012) Biofilm infected intracerebroventricular shunts elicit inflammation within the central nervous system. Infect Immun 80:3206–3214
Paxinos G, Franklin K (2012) The mouse brain in stereotaxic coordinates. Academic, Waltham, MA
Ketamine-xylazine combination for rodent anesthesia. National Cancer Institute Laboratory Animal Sciences Program. http://ncifrederick.cancer.gov/rtp/lasp/intra/acuc/beth/KetamineXylazine.asp. Accessed 28 Dec 2012
Shahrooeil M, Hira V, Stijlemans B et al (2009) Inhibition of Staphylococcus epidermidis biofilm formation by rabbit polyclonal antibodies against the SesC protein. Infect Immun 77:3670–3678
Olsen ME, Slater SR, Rupp ME et al (2010) Rifampicin enhances activity of daptomycin and vancomycin against both a polysaccharide intercellular adhesion (PIA)-dependent and -independent Staphylococcus epidermidis biofilm. J Antimicrob Chemother 65:2164–2171
Kronfrost KD, Mancuso CJ, Pettengill M et al (2012) A neonatal model of intravenous Staphylococcus epidermidis infection in mice, 24 h old, enables characterization of early innate immune responses. PLoS One 7:e43897
Acknowledgements
This work is supported by NIH grant 1K08NS069812-01 and the Cheryl Ann Lozier Memorial Research Fund.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Snowden, J.N. (2014). Generation of a Central Nervous System Catheter-Associated Infection in Mice with Staphylococcus epidermidis . In: Fey, P. (eds) Staphylococcus Epidermidis. Methods in Molecular Biology, vol 1106. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-736-5_18
Download citation
DOI: https://doi.org/10.1007/978-1-62703-736-5_18
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-735-8
Online ISBN: 978-1-62703-736-5
eBook Packages: Springer Protocols