Protocol

Plant Cell Morphogenesis

Volume 1080 of the series Methods in Molecular Biology pp 201-213

Date:

Morphological Analysis of Cell Growth Mutants in Physcomitrella

  • Jeffrey P. BibeauAffiliated withDepartment of Biology and Biotechnology, Worcester Polytechnic institute
  • , Luis VidaliAffiliated withDepartment of Biology and Biotechnology, Worcester Polytechnic institute

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Abstract

This protocol describes a quantitative analysis of the morphology of small plants from the moss Physcomitrella patens. The protocol can be used for the analysis of growth phenotypes produced by transient RNA interference or for the analysis of stable mutant plants. Information is presented to guide the investigator in the choice of vectors and basic conditions to perform transient RNA interference in moss. Detailed directions and examples for fluorescence image acquisition of small regenerating moss plants are provided. Instructions for the use of an ImageJ-based macro for quantitative morphological analysis of these plants are also provided.

Key words

Transient transformation RNAi Physcomitrella patens Morphological analysis Solidity Convex hull Tip growth Cell polarization ImageJ Plant cell growth Cytoskeleton Protonema