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Measurement of Vitamin D

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Hormone Assays in Biological Fluids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1065))

Abstract

There are many different methods to choose for vitamin D analysis. While immunoassays are available commercially and readily automated, specificity for 25-hydroxyvitamin D2 and D3 is variable. Chemical assays such as HPLC and LC-MS/MS have greater specificity and are capable of detecting 25-hydroxyvitamin D2 and D3 separately, but are more complex and require method development. Currently LC-MS/MS is considered the method of choice for 25-hydroxyvitamin D analysis and the methods given here are for LC-MS/MS analysis of serum, plasma and dried whole blood for the separate quantitation of 25-hydroxyvitamin D2 and D3. In addition to highlighting potential interferences, the many non-analytical factors that influence vitamin D measurement are also discussed.

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Authors and Affiliations

  1. Department of Clinical Biochemistry, City Hospital, Birmingham, UK

    Loretta Ford

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  1. Loretta Ford
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Editors and Affiliations

  1. Consultant Clinical Scientist The Old Dairy, Exmouth, United Kingdom

    Michael J. Wheeler

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Ford, L. (2013). Measurement of Vitamin D. In: Wheeler, M. (eds) Hormone Assays in Biological Fluids. Methods in Molecular Biology, vol 1065. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-616-0_16

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  • DOI: https://doi.org/10.1007/978-1-62703-616-0_16

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-615-3

  • Online ISBN: 978-1-62703-616-0

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