Abstract
This chapter describes the measurement of aldosterone concentration in plasma or serum. Aldosterone specificity is improved by first extracting the aldosterone into dichloromethane. A radioimmunoassay is then performed on the dried and reconstituted extract using an anti-aldosterone antibody and iodinated aldosterone. The antibody-bound fraction is separated from the unbound fraction using activated charcoal. The notes section provides extra information for steps in the assay that can be problematic.
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Acknowledgments
With thanks to Mike Scanlon for his expertise and careful review of this and the renin activity chapter.
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Barnes, S.C. (2013). Measurement of Aldosterone in Blood. In: Wheeler, M. (eds) Hormone Assays in Biological Fluids. Methods in Molecular Biology, vol 1065. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-616-0_14
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DOI: https://doi.org/10.1007/978-1-62703-616-0_14
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-615-3
Online ISBN: 978-1-62703-616-0
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