Abstract
As in cancer biology, in wound healing there is a need for objective staging systems to decide for the best treatment and predictors of outcome. We developed in the diabetic (db/db) wound healing model, a staging system, the “wound watch,” based on the quantification of angiogenesis and cell proliferation in open wounds. In chronic wounds, there is often a lack of cellular proliferation and angiogenesis that leads to impaired healing. The wound watch addresses this by quantifying the proliferative phase of wound healing in two dimensions (cellular division and angiogenesis). The results are plotted in a two-dimensional graph to monitor the course of healing and compare the response to different treatments.
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References
Pietramaggiori G, Scherer SS, Mathews JC et al (2008) Healing modulation induced by freeze-dried platelet-rich plasma and micronized allogenic dermis in a diabetic wound model. Wound Repair Regen 16:218–225
Greenhalgh DG (2005) Models of wound healing. J Burn Care Rehabil 26:293–305
Beer HD, Longaker MT, Werner S (1997) Reduced expression of PDGF and PDGF receptors during impaired wound healing. J Invest Dermatol 109:132–138
Lauer G, Sollberg S, Cole M et al (2000) Expression and proteolysis of vascular endothelial growth factor is increased in chronic wounds. J Invest Dermatol 115:12–18
Scherer S, Pietramaggiori G, Mathews J, Chan R, Fiorina P, Orgill DP (2008) Wound healing kinetics of genetically diabetic mouse. Wounds 20(1):18–28
Pietramaggiori G, Kaipainen A, Czeczuga JM, Wagner CT, Orgill DP (2006) Freeze-dried platelet-rich plasma shows beneficial healing properties in chronic wounds. Wound Repair Regen 14:573–580
Chan RK, Liu PH, Pietramaggiori G, Ibrahim SI, Hechtman HB, Orgill DP (2006) Effect of recombinant platelet-derived growth factor (Regranex) on wound closure in genetically diabetic mice. J Burn Care Res 27:202–205
Pietramaggiori G, Scherer SS, Cervi D, Klement G, Orgill DP (2008) Tumors stimulate platelet delivery of angiogenic factors in vivo: an unexpected benefit. Am J Pathol 173:1609–1616
Pietramaggiori G, Scherer SS, Mathews JC et al (2010) Quiescent platelets stimulate angiogenesis and diabetic wound repair. J Surg Res 160:169–177
Fiorina P, Pietramaggiori G, Scherer SS et al (2010) The mobilization and effect of endogenous bone marrow progenitor cells in diabetic wound healing. Cell Transplant 19:1369–1381
Scherer SS, Pietramaggiori G, Mathews JC, Prsa MJ, Huang S, Orgill DP (2008) The mechanism of action of the vacuum-assisted closure device. Plast Reconstr Surg 122:786–797
Pietramaggiori G, Kaipainen A, Ho D et al (2007) Trehalose lyophilized platelets for wound healing. Wound Repair Regen 15:213–220
Scherer SS, Pietramaggiori G, Matthews J et al (2009) Poly-N-acetyl glucosamine nanofibers: a new bioactive material to enhance diabetic wound healing by cell migration and angiogenesis. Ann Surg 250:322–330
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Pietramaggiori, G., Scherer, S., Orgill, D.P. (2013). The Wound Watch: An Objective Staging System for Wounds in the Diabetic (db/db) Mouse Model. In: Gourdie, R., Myers, T. (eds) Wound Regeneration and Repair. Methods in Molecular Biology, vol 1037. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-505-7_13
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DOI: https://doi.org/10.1007/978-1-62703-505-7_13
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Publisher Name: Humana Press, Totowa, NJ
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