Protocol

NanoBiotechnology Protocols

Volume 1026 of the series Methods in Molecular Biology pp 175-186

Date:

In Vivo Testing for Gold Nanoparticle Toxicity

  • Carrie A. SimpsonAffiliated withUniversity of Colorado
  • , Brian J. HuffmanAffiliated withUniversity of North Alabama
  • , David E. CliffelAffiliated withVanderbilt University

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Abstract

A technique for measuring the toxicity of nanomaterials using a murine model is described. Blood samples are collected via submandibular bleeding while urine samples are collected on cellophane sheets. Both biosamples are then analyzed by inductively coupled plasma optical emission spectroscopy (ICP-OES) for nanotoxicity. Blood samples are further tested for immunological response using a standard Coulter counter. The major organs of interest for filtration are also digested and analyzed via ICP-OES, producing useful information regarding target specificity of the nanomaterial of interest. Collection of the biosamples and analysis afterward is detailed, and the operation of the technique is described and illustrated by analysis of the nanotoxicity of an injection of a modified tiopronin monolayer-protected cluster.

Key words

Nanotoxicity Murine model Biodistribution Tissue analysis Organ digestion