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Vascular Proteomics

Volume 1000 of the series Methods in Molecular Biology pp 157-166

Date:

Unraveling Biomarkers of Abdominal Aortic Aneurisms by iTRAQ Analysis of Depleted Plasma

  • Enrique CalvoAffiliated withUnidad de Proteómica, Centro Nacional de Investigaciones Cardiovasculares, CNIC
  • , Roxana Martínez-PinnaAffiliated withVascular Research Lab IIS-Fundación Jiménez Díaz, Instituto de Investigacion Sanitaria Fundacion Jimenez Diaz (IIS-FJD), Universidad Autónoma de Madrid
  • , Priscila Ramos-MozoAffiliated withVascular Research Laboratory, IIS, Fundación Jiménez Díaz ands Autónoma University
  • , C. Pastor-VargasAffiliated withDepartment of Immunology, Instituto de Investigacion Sanitaria Fundacion Jimenez Diaz (IIS-FJD)
  • , Emilio CamafeitaAffiliated withUnidad de Proteómica, Centro Nacional de Investigaciones Cardiovasculares, CNIC
  • , Jesús EgidoAffiliated withVascular Research Lab IIS-Fundación Jiménez Díaz, Instituto de Investigacion Sanitaria Fundacion Jimenez Diaz (IIS-FJD), Universidad Autónoma de Madrid
  • , José Luis Martin-VenturaAffiliated withVascular Research Lab IIS-Fundación Jiménez Díaz, Instituto de Investigacion Sanitaria Fundacion Jimenez Diaz (IIS-FJD), Universidad Autónoma de Madrid
  • , Juan Antonio LopezAffiliated withUnidad de Proteómica, Centro Nacional de Investigaciones Cardiovasculares, CNIC

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Abstract

Abdominal aortic aneurysm (AAA) is a significant health problem in Western countries. The diameter of AAA is a surrogate marker of its growth rate that reflects the magnitude of the degenerative process in the vascular wall, although most AAAs show discontinuous growth patterns and alternate periods of stability and nongrowth with periods of acute expansion and occasionally ruptures. Thus, the identification of biomarkers of AAA in plasma could aid in the diagnosis, prognosis, and therapy of AAA progression. However, owing to the complex composition of plasma, depletion methods must be applied before the analytical approaches for detecting low-abundant plasma protein components. In the present work, we describe a proteomic study on MARS 14-depleted plasma based on mass spectrometry (MS) which combines peptide labelling (isobaric tagging for relative and absolute quantification, iTRAQ) and liquid chromatography–tandem mass spectrometry (LC–MS/MS). This quantitative approach revealed altered levels of several proteins related to the complement system in AAA patients.

Key words

Abdominal aortic aneurysm iTRAQ Mass spectrometry Proteomics Depleted plasma Biomarkers