Abstract
We describe various complementary techniques to achieve multidimensional mitochondrial proteome fractionation and analysis. Previously described methods for 2D-DIGE/mass spectrometry and 1D-SDS-PAGE/Western techniques and protein complex analysis by BN-PAGE/Western and sucrose gradient ultracentrifugation/SDS-PAGE/mass spectrometry are optimized to characterize the brain mitochondrial proteome. This approach allows for a comprehensive identification of mitochondrial proteomic differences between health and disease conditions.
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Lopez-Campistrous, A., Fernandez-Patron, C. (2013). Proteomic Analysis of Brain Mitochondrial Proteome and Mitochondrial Complexes. In: Vivanco, F. (eds) Heart Proteomics. Methods in Molecular Biology, vol 1005. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-386-2_11
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DOI: https://doi.org/10.1007/978-1-62703-386-2_11
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-385-5
Online ISBN: 978-1-62703-386-2
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