Abstract
Mantle cell lymphoma (MCL) is characterized by the presence of t(11;14)(q13;q32) which juxtaposes CCND-1 gene (also known as BCL-1, PRAD-1) at 11q13 with an enhancer of the IGH@ gene at 14q32. The resultant overexpression of cyclin D1 plays an essential role in the pathogenesis of MCL. The breakpoints on chromosome 14 occur 5′ to one of six JH segments, whereas only 30–50% of the breakpoints on chromosome 11 are localized within a 1 kb region called the major translocation cluster (MTC) which can be easily assessed by polymerase chain reaction (PCR). The remainder of the breakpoints are widely scattered over approximately 120 kb, making PCR analysis infeasible. We describe a TaqMan-based real-time PCR assay to detect and quantify IGH@/BCL1 fusion products in newly diagnosed MCL, and to monitor minimal residual disease during treatment or early relapse in MTC-positive cases.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Swerdlow SH, Campo E, Seto M, Muller-Hermelink HK (2008) Mantle cell lymphoma. In: Swerdlow SH, Campo E, Harris NL, Jaffe ES, Pileri SA, Stein H et al (eds) WHO classification of tumours of haematopoietic and lymphoid tissues. IARC, Lyon, pp 229–232
Lorsbach RB, His ED, Dogan A, Fend F (2011) Plasma cell myeloma and related neoplasms. Am J Clin Pathol 136:168–182
Yin CC (2011) Mantle cell lymphoma. In: Medeiros LJ (ed) Diagnostic pathology. Lymph nodes and spleen with extranodal lymphomas, Amirsys Publishing, Inc. pp 6:42–45
Williams ME, Swerdlow SH, Rosenberg CL, Arnold A (1993) Chromosome 11 translocation breakpoints at the PRAD1/cyclin D1 gene locus in centrocytic lymphoma. Leukemia 7:241–245
Luthra R, Hai S, Pugh WC (1995) Polymerase chain reaction detection of the t(11;14) translocation involving the bcl-1 major translocation cluster in mantle cell lymphoma. Diagn Mol Pathol 4:4–7
Luthra R, Sarris AH, Hai S, Paladugu AV, Romaguera JE, Cabanilla FF et al (1999) Real-time 5′–3′ exonuclease-based PCR assay for detection of the t(11;14)(q13;q32). Am J Clin Pathol 112:524–530
Medeiros LJ, Hai S, Thomazy VA, Estalilla OC, Romaguera J, Luthra R (2002) Real-time RT-PCR assay for quantifying cyclin D1 mRNA in B-cell non-Hodgkin’s lymphomas. Mod Pathol 15:556–564
Hui P, Howe JG, Crouch J, Nimmakayalu M, Qumsiyeh MB, Tallini G et al (2003) Real-time quantitative RT-PCR of cyclin D1 mRNA in mantle cell lymphoma: comparison with FISH and immunohistochemistry. Leuk Lymphoma 44:1385–1394
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media, New York
About this protocol
Cite this protocol
Yin, C.C., Luthra, R. (2013). Molecular Detection of t(11;14)(q13;q32) in Mantle Cell Lymphoma. In: Czader, M. (eds) Hematological Malignancies. Methods in Molecular Biology, vol 999. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-357-2_14
Download citation
DOI: https://doi.org/10.1007/978-1-62703-357-2_14
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-356-5
Online ISBN: 978-1-62703-357-2
eBook Packages: Springer Protocols