Abstract
This chapter introduces atomic force microscopy (AFM) as an important tool for protein nanotechnology. A short review of AFM-based imaging, mapping, and spectroscopy of protein samples is given. AFM imaging of β-lactoglobulin nanofibrils in air is demonstrated. Basic concepts of AFM are described. Protocols for β-lactoglobulin nanofibrils and multiwall carbon nanotubes (MWCNT) samples preparation are defined. The operation of the microscope is described using MWCNT and the NanoScope E instrument in contact mode as an example. Nanostructure manipulation based on AFM nano-sweeping is demonstrated.
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Acknowledgements
The author would like to thank Professor Richard Haverkamp, School of Engineering and Advanced Technology, and Dr. Mark Waterland, Institute for Fundamental Sciences, Massey University, for their support and opportunity to work with scanning probe microscopes. The author would also like to thank Dr. Mark Patchett, Institute of Molecular BioSciences, for his valuable suggestions, and Dr. Simon Loveday, Riddet Institute, and Mike Seawright, Institute for Fundamental Sciences, Massey University, for supplying protein and nanotubes samples and technical assistance with sample preparation for AFM.
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Sokolov, D.V. (2013). Atomic Force Microscopy for Protein Nanotechnology. In: Gerrard, J. (eds) Protein Nanotechnology. Methods in Molecular Biology, vol 996. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-354-1_19
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DOI: https://doi.org/10.1007/978-1-62703-354-1_19
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