Abstract
In this chapter, we describe the steps needed to create a kinetic model of a metabolic pathway using kinetic data from both experimental measurements and literature review. Our methodology is presented by using the example of serine biosynthesis in E. coli.
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Acknowledgements
KS is grateful for the financial support of the EU FP7 (KBBE) grant 289434 “BioPreDyn: New Bioinformatics Methods and Tools for Data-Driven Predictive Dynamic Modelling in Biotechnological Applications.” We thank Daniel Jameson for taking the time to comment on the manuscript. Natalie Stanford acknowledges the support of DirectFuel, a European Union Seventh Framework Programme (FP7-ENERGY-2010-1) under grant agreement n. [256808].
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Smallbone, K., Stanford, N.J. (2013). Kinetic Modeling of Metabolic Pathways: Application to Serine Biosynthesis. In: Alper, H. (eds) Systems Metabolic Engineering. Methods in Molecular Biology, vol 985. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-299-5_7
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DOI: https://doi.org/10.1007/978-1-62703-299-5_7
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Publisher Name: Humana Press, Totowa, NJ
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