Abstract
Capillary electrophoresis (CE) of chitooligosaccharides (COS) in aqueous solution is effective for their separation from other saccharides. However, COS easily adsorb on negatively charged surfaces, such as fused silica capillaries in acidic solutions. Conventional photometric detection cannot be applied directly because saccharides do not absorb ultraviolet or visible light. Here, we describe a simple CE of COS in an acidic solution using a positively charged capillary coated with N-trimethoxypropyl-N,N,N-trimethylammonium chloride and indirect photometric detection with crystal violet nitrate background solution.
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References
Dong Q, Stellwagen E, Dagle JM, Stellwagen NC (2003) Free solution mobility of small Âsingle-stranded oligonucleotides with variable charge densities. Electrophoresis 24:3323–3329
Hoagland DA, Arvanitidou E, Welch C (1999) Capillary electrophoresis measurements of the free solution mobility for several model polyelectrolyte systems. Macromolecules 32:6180–6190
Cottet H, Gareil P (2000) From small charged molecules to oligomers: a semiempirical approach to the modeling of actual mobility in free solution. Electrophoresis 21:1493–1504
Altria KD (1996) Capillary electrophoresis guidebook: principles, operation, and applications. Method Mol Biol 52:9
Blanes L, Saito RM, Genta FA, Donegá J, Terra WR, Ferreira C, do Lago CL (2008) Direct detection of underivatized chitooligosaccharides produced through chitinase action using capillary zone electrophoresis. Anal Biochem 373:99–103
Katayama H, Ishihama Y, Asakawa N (1998) Stable cationic capillary coating with successive multiple ionic polymer layers for capillary electrophoresis. Anal Chem 70:5272–5277
Hattori T, Anraku N, Kato R (2010) Capillary electrophoresis of chitooligosaccharides in acidic solution: its simple determination method using quaternary-ammonium-modified column and indirect photometric detection with Crystal Violet. J Chromatogr B 878:477–480
Beaudoin M-E, Gauthier J, Boucher I, Waldron KC (2005) Capillary electrophoresis separation of a mixture of chitin and chitosan oligosaccharides derivatized using a modified fluorophore conjugation procedure. J Sep Sci 28:1390–1398
Izume M, Ohtakara A (1987) Preparation of d-glucosamine oligosaccharides by the enzymatic hydrolysis of chitosan. Agric Biol Chem 51:1189–1191
Uchida Y, Ohtakara A (1988) Chitosanase from Bacillus species. Methods Enzymol 161:501–505
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Hattori, T., Anraku, N., Kato, R. (2013). Separation of Chitooligosaccharides in Acidic Solution by Capillary Electrophoresis. In: Volpi, N., Maccari, F. (eds) Capillary Electrophoresis of Biomolecules. Methods in Molecular Biology, vol 984. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-296-4_6
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DOI: https://doi.org/10.1007/978-1-62703-296-4_6
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Publisher Name: Humana Press, Totowa, NJ
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