Abstract
Proper lymphocyte apoptosis is critical for the maintenance of immune system homeostasis, and evaluation of cell death is useful in a number of clinical and research settings. We describe here how to evaluate the integrity of the intrinsic pathway of lymphocyte apoptosis triggered by starvation for IL-2 or other survival cytokines.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Lenardo M, Chan KM, Hornung F et al (1999) Mature T lymphocyte apoptosis-immune Âregulation in a dynamic and unpredictable antigenic environment. Annu Rev Immunol 17: 221–253
Tibbetts MD, Zheng L, Lenardo MJ (2003) The death effector domain protein family: regulators of cellular homeostasis. Nat Immunol 4(5):404–409
Bidere N, Su HC, Lenardo MJ (2006) Genetic disorders of programmed cell death in the immune system. Annu Rev Immunol 24: 321–352
Strasser A (2005) The role of BH3-only proteins in the immune system. Nat Rev Immunol 5(3):189–200
Oliveira JB, Bidere N, Niemela JE et al (2007) NRAS mutation causes a human autoimmune lymphoproliferative syndrome. Proc Natl Acad Sci U S A 104(21):8953–8958
Niemela JE, Lu L, Fleisher TA et al. Somatic KRAS mutations associated with a human nonmalignant syndrome of autoimmunity and abnormal leukocyte homeostasis. Blood. 10; 117(10):2883–2886.
Strasser A, Pellegrini M (2004) T-lymphocyte death during shutdown of an immune response. Trends Immunol 25(11):610–615
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media New York
About this protocol
Cite this protocol
Oliveira, J.B. (2013). Evaluation of IL-2-Withdrawal-Induced Apoptosis in Human T Lymphocytes. In: Snow, A., Lenardo, M. (eds) Immune Homeostasis. Methods in Molecular Biology, vol 979. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-290-2_3
Download citation
DOI: https://doi.org/10.1007/978-1-62703-290-2_3
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-289-6
Online ISBN: 978-1-62703-290-2
eBook Packages: Springer Protocols