Abstract
A comprehensive identification of protein–DNA interactions that drive processes such as transcription and replication, both in prokaryotic and eukaryotic organisms, remains a major technical challenge. In this chapter, we present a SILAC-based DNA affinity purification method that can be used to identify specific interactions between proteins and functional DNA elements in an unbiased manner.
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Acknowledgments
We thank members of the Vermeulen lab for critical reading of the manuscript. The Vermeulen lab is supported by grants from the Netherlands organization for scientific Research (NWO-VIDI), the Dutch Cancer Society (KWF), and the Cancer Genomics Centre (CGC-NGI).
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Spruijt, C.G., Baymaz, H.I., Vermeulen, M. (2013). Identifying Specific Protein–DNA Interactions Using SILAC-Based Quantitative Proteomics. In: Bina, M. (eds) Gene Regulation. Methods in Molecular Biology, vol 977. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-284-1_11
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DOI: https://doi.org/10.1007/978-1-62703-284-1_11
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