Abstract
Plant viral vectors are useful for transient gene expression as well as for downregulation of gene expression via virus-induced gene silencing (VIGS). When used in reverse genetics approaches, VIGS offers a convenient way of transforming genomic information into knowledge of gene function. Efforts to develop and improve plant viral vectors have expanded their applications and have led to substantial advances needed to facilitate gene function studies in major row crops. Here, we describe a DNA-based Bean pod mottle virus (BPMV) vector system for both gene expression and VIGS in soybean and common bean.
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Acknowledgments
This work was supported by the Iowa State University Plant Sciences Institute Innovation Research Grants Program, the North Central Soybean Research Program, the Iowa Soybean Association, the United Soybean Board, the National Science Foundation Plant Genome Research Program (grant no. 0820642), and Hatch Act and State of Iowa funds.
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Zhang, C., Whitham, S.A., Hill, J.H. (2013). Virus-Induced Gene Silencing in Soybean and Common Bean. In: Becker, A. (eds) Virus-Induced Gene Silencing. Methods in Molecular Biology, vol 975. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-278-0_11
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DOI: https://doi.org/10.1007/978-1-62703-278-0_11
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-277-3
Online ISBN: 978-1-62703-278-0
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