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Solid-State NMR Approaches to Study Protein Structure and Protein–Lipid Interactions

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Book cover Lipid-Protein Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 974))

Abstract

Solid-state NMR spectroscopy has been developed for the investigation of membrane-associated polypeptides and remains one of the few techniques to reveal high-resolution structural information in liquid-disordered phospholipid bilayers. In particular, oriented samples have been used to investigate the structure, dynamics, and topology of membrane polypeptides. Much of the previous solid-state NMR work has been developed and performed on peptides, but the technique is constantly expanding towards larger membrane proteins. Here, a number of protocols are presented describing among other the reconstitution of membrane proteins into oriented membranes, monitoring membrane alignment by 31P solid-state NMR spectroscopy; investigations of the protein by one- and two-dimensional 15N solid-state NMR; and measurements of the lipid order parameters using 2H solid-state NMR spectroscopy. Using such methods solid-state NMR spectroscopy has revealed a detailed picture of the ensemble of both lipids and proteins and their mutual interdependence in the bilayer environment.

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Acknowledgements

The help and comments by Jesus Raya and Philippe Bertani on the nutation and PISEMA experiments are gratefully acknowledged. We are grateful for the financial support by the Deutsche Forschungsgemeinschaft (postdoctoral grant to MM), the University of Strasbourg (PPF RMN), the CNRS (NMR equipment), and the Chemistry Institute (UMR7177).

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Authors and Affiliations

  1. Institut de chimie, CNRS, Université de Strasbourg, UMR 7177, Strasbourg, France

    Christopher Aisenbrey, Matthias Michalek, Evgeniy S. Salnikov & Burkhard Bechinger

Authors
  1. Christopher Aisenbrey
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  2. Matthias Michalek
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  3. Evgeniy S. Salnikov
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  4. Burkhard Bechinger
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Corresponding author

Correspondence to Burkhard Bechinger .

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Editors and Affiliations

  1. Institut für Biologie, Abteilung Biophysik, Universität Kassel, Heinrich-Plett-Straße 40, Kassel, 34132, Germany

    Jörg H. Kleinschmidt

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Aisenbrey, C., Michalek, M., Salnikov, E.S., Bechinger, B. (2013). Solid-State NMR Approaches to Study Protein Structure and Protein–Lipid Interactions. In: Kleinschmidt, J. (eds) Lipid-Protein Interactions. Methods in Molecular Biology, vol 974. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-275-9_16

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  • DOI: https://doi.org/10.1007/978-1-62703-275-9_16

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-274-2

  • Online ISBN: 978-1-62703-275-9

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