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Myelin Proteome Analysis: Methods and Implications for the Myelin Cytoskeleton

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The Cytoskeleton

Part of the book series: Neuromethods ((NM,volume 79))

Abstract

Myelin, the multilayered membrane that enwraps and insulates neuronal axons for fast signal propagation, is a plasma membrane specialization of oligodendrocytes and Schwann cells in the central and peripheral nervous system, respectively. Here we provide our lab protocols for the purification of myelin from mouse brains and for gel-based and gel-free proteomic applications, as well as a brief discussion with respect to our current knowledge of the myelin cytoskeleton.

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Acknowledgments

We thank N. Schaeren-Wiemers and K.-I. Nagata for antibody gifts, D. Hesse, T. Liepold, and M. Uecker for proof-reading, and K.-A. Nave for continuous support. Our work is supported by the Bundesministerium für Bildung und Forschung (BMBF DLR-Leukonet), the European Commission (EU-Health FP7 LeukoTreat), and the Deutsche Forschungsgemeinschaft (DFG-SFB490, Z3).

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Authors and Affiliations

  1. Max Planck Institute of Experimental Medicine, Göttingen, Germany

    Olaf Jahn, Nicole Bartsch, Julia Patzig & Hauke B. Werner

  2. University Medical Center, Johannes Gutenberg University Mainz, Mainz, Germany

    Stefan Tenzer

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  1. Olaf Jahn
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  2. Stefan Tenzer
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  3. Nicole Bartsch
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  4. Julia Patzig
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Correspondence to Hauke B. Werner .

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  1. Faculty of Medicine, Neuroanatomy & Molecular Brain Research, Ruhr-Universität Bochum, Universitätsstrasse 150, Bochum, 44801, Germany

    Rolf Dermietzel

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Jahn, O., Tenzer, S., Bartsch, N., Patzig, J., Werner, H.B. (2013). Myelin Proteome Analysis: Methods and Implications for the Myelin Cytoskeleton. In: Dermietzel, R. (eds) The Cytoskeleton. Neuromethods, vol 79. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-266-7_15

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  • DOI: https://doi.org/10.1007/978-1-62703-266-7_15

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