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Acetylation of Endogenous STAT Proteins

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JAK-STAT Signalling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 967))

Abstract

Acetylation of signal transducer and activator of transcription (STAT) proteins has been recognized as a significant mechanism for the regulation of their cellular functions. Site-specific antibodies are available only for a minority of STATs. The detection of acetylated STATs by immunoprecipitation (IP) followed by western blot (WB) will be described in the following chapter. Defined conditions for cell lysis and IP will be elucidated on the basis of STAT1 acetylation.

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Authors and Affiliations

  1. Center for Molecular Biomedicine (CMB), Department of Biochemistry, University of Jena, Jena, Germany

    Torsten Ginter, Thorsten Heinzel & Oliver H. Krämer

Authors
  1. Torsten Ginter
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  2. Thorsten Heinzel
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  3. Oliver H. Krämer
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Corresponding author

Correspondence to Oliver H. Krämer .

Editor information

Editors and Affiliations

  1. Walter and Eliza Hall Institute, Royal Parade 1G, Parkville, 3050, Victoria, Australia

    Sandra E. Nicholson

  2. Walter and Eliza Hall Institute, Royal Parade 1G, Parkville, 3050, Victoria, Australia

    Nicos A. Nicola

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Ginter, T., Heinzel, T., Krämer, O.H. (2013). Acetylation of Endogenous STAT Proteins. In: Nicholson, S., Nicola, N. (eds) JAK-STAT Signalling. Methods in Molecular Biology, vol 967. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-242-1_12

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  • DOI: https://doi.org/10.1007/978-1-62703-242-1_12

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-241-4

  • Online ISBN: 978-1-62703-242-1

  • eBook Packages: Springer Protocols

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