Abstract
Flow cytometry can be used to study STAT phosphorylation on a per cell basis. Cells are fixed, permeablized, and stained with antibodies that specifically bind to the phosphorylated form of the STAT protein. This allows the tyrosine phosphorylation of a single STAT to be studied within a heterogeneous cell population and/or the phosphorylation of several STATs within the one cell type.
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References
Perez OD, Nolan GP (2006) Phospho-proteomic immune analysis by flow cytometry: from mechanism to translational medicine at the single-cell level. Immunol Rev 210:208–228
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Acknowledgments
Gabrielle Goldberg is an NHMRC Career Development Fellow. This work was made possible through Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS.
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Murphy, J., Goldberg, G.L. (2013). Flow Cytometric Analysis of STAT Phosphorylation. In: Nicholson, S., Nicola, N. (eds) JAK-STAT Signalling. Methods in Molecular Biology, vol 967. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-242-1_11
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DOI: https://doi.org/10.1007/978-1-62703-242-1_11
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-241-4
Online ISBN: 978-1-62703-242-1
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