Protocol

p53 Protocols

Volume 962 of the series Methods in Molecular Biology pp 31-48

Date:

Autophagy, Senescence, and Apoptosis

  • Rachel W. GoeheAffiliated withDepartment of Pharmacology and Toxicology, Virginia Commonwealth UniversityDepartment of Radiation Oncology, Virginia Commonwealth University
  • , Molly L. BristolAffiliated withDepartment of Pharmacology and Toxicology, Virginia Commonwealth UniversityDepartment of Pathology, Virginia Commonwealth University
  • , Eden N. WilsonAffiliated withDepartment of Pharmacology and Toxicology, Virginia Commonwealth University
  • , David A. GewirtzAffiliated withMassey Cancer Center, Virginia Commonwealth University Email author 

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Abstract

This chapter presents methods for interrogating the involvement of p53 in signaling to apoptosis, autophagy, and senescence. The well-known association of p53 with the stress response to chemotherapy and radiation is the basis for presenting these approaches. The development of quantitative and efficient in vitro assays has enabled researchers to overcome the limitations of previous methodologies. This chapter provides up-to-date procedures relating to the molecular networks in which the p53 protein has been shown to play a central role that allows damaged cells either to adapt to stress (autophagy and/or senescence) or to progress towards programmed cell death (apoptosis).

Key words

Apoptosis Senescence Autophagy Flow cytometry Western immunoblotting Fluorescent microscopy Acridine orange β-Galactosidase Annexin V Propidium iodide