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Molecular Diagnosis of Autoimmune Blistering Diseases

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Molecular Dermatology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 961))

Abstract

Autoimmune bullous diseases are the best-characterized autoimmune skin diseases. Molecular diagnosis of these diseases has become possible due to the identification of their target autoantigens over the past three decades. In this review, we summarize methodology for categorizing autoimmune bullous diseases by means of combinations of direct and indirect immunofluorescence techniques using normal human skin sections, rat bladder sections and COS7 cells transfected with desmocollins 1–3 encoded vectors, enzyme-linked immunosorbent assays and immunoblotting with normal human epidermal extracts, dermal extracts, purified proteins from cell cultures and recombinant proteins.

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Acknowledgment

We greatly appreciate Ms. Ayumi Suzuki, Ms. Takako Ishikawa, and Ms. Sachiko Sakaguchi for technical assistance, and Ms. Akiko Tanaka, Ms. Yasuko Nakayama, Ms. Emiko Hara, Ms. Hanako Tomita, Ms. Mihoko Ikeda, Ms. Kyoko Akashi, and Ms. Nobuko Ishii for secretarial work.

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Authors and Affiliations

  1. Department of Dermatology, School of Medicine, and Institute of Cutaneous Cell Biology, Kurume University, Kurume, Fukuoka, Japan

    Daisuke Tsuruta, Teruki Dainichi & Takahiro Hamada

  2. Department of Dermatology, School of Medicine, Kurume University, Kurume, Fukuoka, Japan

    Norito Ishii

  3. Institute of Cutaneous Cell Biology, Kurume University, Kurume, Fukuoka, Japan

    Norito Ishii & Takashi Hashimoto

  4. Department of Dermatology, Molecular Dermatology, School of Medicine, Kurume University, Kurume, Fukuoka, Japan

    Takashi Hashimoto

Authors
  1. Daisuke Tsuruta
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  2. Teruki Dainichi
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  3. Takahiro Hamada
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  4. Norito Ishii
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  5. Takashi Hashimoto
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Corresponding author

Correspondence to Takashi Hashimoto .

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Editors and Affiliations

  1. Universitäts-Hautklinik, Department of Molecular Dermatology, Universitätsklinikum Freiburg, Hauptstr. 7, Freiburg, 79104, Germany

    Cristina Has

  2. Universitäts-Hautklinik, Department of Molecular Dermatology, Universitätklinikum Freiburg, Hauptstr. 7, Freiburg, 79104, Germany

    Cassian Sitaru

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Tsuruta, D., Dainichi, T., Hamada, T., Ishii, N., Hashimoto, T. (2013). Molecular Diagnosis of Autoimmune Blistering Diseases. In: Has, C., Sitaru, C. (eds) Molecular Dermatology. Methods in Molecular Biology, vol 961. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-227-8_2

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  • DOI: https://doi.org/10.1007/978-1-62703-227-8_2

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