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Combination of In Vivo Microdialysis with Selective Electrochemical Detection for Online Continuous Monitoring of Brain Chemistry

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Microdialysis Techniques in Neuroscience

Part of the book series: Neuromethods ((NM,volume 75))

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Abstract

This chapter summarizes the recent development of combination of in vivo microdialysis with selective detection, especially electrochemical detection, to form novel online analytical methods for continuously monitoring brain chemistry, without the need for sample collection, pretreatment, or separation. While efficient combination of in vivo microdialysis sampling directly with selective electrochemical detection is envisaged to provide less technically demanding in vivo and online analytical methods for near-real-time monitoring physiologically important neurochemicals in the living animals, which is particularly useful for understanding the molecular basis of brain functions, the complexity in the components of the cerebral systems and the inherent features of the as-developed integrated online analytical systems (i.e., without sampling collection, pretreatment, or separation) virtually puts the selective electrochemical detection into a great challenge with respect to the selectivity, sensitivity, stability, and reproducibility. This chapter focuses on the following three aspects: (1) brief introduction of in vivo and online analytical methods developed by directly combining in vivo microdialysis sampling with selective electrochemical detection; (2) recent developments along with this line both in the method establishment and in their applications in understanding brain chemistry through interfacing electroanalytical chemistry with physiology and pathology; and (3) summary and future developments in this field.

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Acknowledgement

This work is financially supported by NSF of China (Grant Nos., 90813032, 20935005, 20975104 for L. Mao, and 21045001 for Y. Lin), National Basic Research Program of China (973 Program, 2007CB935603 and 2010CB933502), and Chinese Academy of Sciences (KJCX2-YW-W25, Y2010015).

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Authors and Affiliations

  1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, The Chinese Academy of Sciences (CAS), Beijing, China

    Yuqing Lin & Lanqun Mao

  2. Key Laboratory of Analytical Chemistry for Living Biosystems, Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, The Chinese Academy of Sciences (CAS), Beijing, China

    Zipin Zhang

Authors
  1. Yuqing Lin
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  2. Zipin Zhang
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  3. Lanqun Mao
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Correspondence to Lanqun Mao .

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Editors and Affiliations

  1. , Dept. of Physiology & Biochemistry, University of Malta, Biomedical Sciences Building, Msida MSD, 2080, Malta

    Giuseppe Di Giovanni

  2. Consorzio "Mario Negri" Sud, Via Nazionale, Santa Maria Imbaro, 66030, Chieti, Italy

    Vincenzo Di Matteo

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Lin, Y., Zhang, Z., Mao, L. (2013). Combination of In Vivo Microdialysis with Selective Electrochemical Detection for Online Continuous Monitoring of Brain Chemistry. In: Di Giovanni, G., Di Matteo, V. (eds) Microdialysis Techniques in Neuroscience. Neuromethods, vol 75. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-173-8_7

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  • DOI: https://doi.org/10.1007/978-1-62703-173-8_7

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-172-1

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