Abstract
Metabolic pathways and fluxes can be analyzed under in vivo conditions by incorporation experiments using general 13C-labeled precursors. On the basis of the isotopologue compositions in amino acids or other metabolites, the incorporation rates of the supplied precursors and the pathways of their utilization can be studied in considerable detail. In this chapter, the method of isotopologue profiling is illustrated with recent work on the metabolism of intracellular living Legionella pneumophila.
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Acknowledgments
We thank our coworkers Nadine Gillmaier, Vroni Herrmann, Eva Eylert, Claudia Huber, Birgit Lange, and Eva Schunder for their enthusiastic help in establishing and optimizing the methods described in this article. This work was financed by grants from the Deutsche Forschungsgemeinschaft DFG SPP 1316 (Bonn, Germany) (EI 384/4-1 and HE 2845/6-1, respectively).
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Heuner, K., Eisenreich, W. (2013). The Intracellular Metabolism of Legionella by Isotopologue Profiling. In: Buchrieser, C., Hilbi, H. (eds) Legionella. Methods in Molecular Biology, vol 954. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-161-5_8
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DOI: https://doi.org/10.1007/978-1-62703-161-5_8
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