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Typing Methods for Legionella

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Legionella

Part of the book series: Methods in Molecular Biology ((MIMB,volume 954))

Abstract

In this chapter we describe the methods currently used for subgrouping Legionella pneumophila and other non-pneumophila species. In the first part we describe monoclonal antibody (mAb) subgrouping, either by indirect immunofluorescence or indirect ELISA methods. These monoclonal antibodies are not commercially available but can be obtained for noncommercial purposes from one of the authors. Further, we describe pulsed-field gel electrophoresis (PFGE), amplified fragment length polymorphism (AFLP) and sequence-based typing (SBT) as well standardized and reproducible methods for genotyping. The SBT schema is currently available for L. pneumophila whereas PFGE and AFLP can be used for all Legionella species. For certain applications it might be useful to use spoligotyping to distinguish strains belonging to the same sequence type (ST).

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Author information

Authors and Affiliations

  1. Institute for Medical Microbiology and Hygiene, University of Technology, Dresden, Germany

    Christian Lück & Jürgen H. Helbig

  2. Respiratory and Systemic Infection Laboratory, Health Protection Agency, London, UK

    Norman K. Fry & Timothy G. Harrison

  3. National Reference Center of Legionella, Centre de Biologie Est, Hospices Civils de Lyon, Lyon, France

    Sophie Jarraud

  4. Université de Lyon, Lyon, France

    Sophie Jarraud

Authors
  1. Christian Lück
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  2. Norman K. Fry
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  3. Jürgen H. Helbig
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  4. Sophie Jarraud
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  5. Timothy G. Harrison
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Corresponding author

Correspondence to Christian Lück .

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Editors and Affiliations

  1. , Biology of Intracellular Bacteria, Institut Pasteur, Rue du Dr. Roux 28, Paris, 75724, France

    Carmen Buchrieser

  2. Max von Pettenkofer Institute, Ludwig-Maximilians-University, Pettenkoferstrasse 9a, Munich, 80336, Germany

    Hubert Hilbi

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Lück, C., Fry, N.K., Helbig, J.H., Jarraud, S., Harrison, T.G. (2013). Typing Methods for Legionella . In: Buchrieser, C., Hilbi, H. (eds) Legionella. Methods in Molecular Biology, vol 954. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-161-5_6

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  • DOI: https://doi.org/10.1007/978-1-62703-161-5_6

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-160-8

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