Abstract
Fluorescence in situ hybridization (FISH) is increasingly gaining importance in clinical diagnostics settings. Due to the ability of the technique to detect chromosomal abnormalities in samples with low cellularity or containing a mixed population of cells even at a single-cell level, it has become more popular in cancer research and diagnosis. Here, we describe the FISH technique for detection of PAX8-PPARγ translocation in follicular thyroid neoplasms, and the optimal protocol for the detection of this fusion gene using in archival formalin-fixed paraffin-embedded (FFPE) thyroid tissue sections.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Kroll TG, Sarraf P, Pecciarini L et al (2000) PAX8-PPARγ1 fusion oncogene in human thyroid carcinoma. Science 289:1357–1360
Lui WO, Foukakis T, Liden J et al (2004) Expression profiling reveals a distinct transcription signature in follicular thyroid carcinomas with a PAX8-PPARγ fusion oncogene. Oncogene 24:1467–1476
Liehr T, Manvelyan M (2009) Characterization of archived formalin-fixed/paraffin-embedded or cryofixed tissue, including nucleus extraction. In: Liehr T (ed) Fluorescence in situ hybridization (FISH)—application guide. Springer, Berlin, Heidelberg, Germany, pp 147–156
Ventura RA, Martin-Subero J, Jones M et al (2006) FISH analysis for the detection of lymphoma-associated chromosomal abnormalities in routine paraffin-embedded tissue. J Mol Diagn 8:141–151
Chia WK, Sharifah N, Reena R et al (2010) Fluorescence in situ hybridization analysis using PAX8- and PPARG-specific probes reveals the presence of PAX8-PPARG translocation and 3p25 aneusomy in follicular thyroid neoplasms. Cancer Genet Cytogenet 196:7–13
Pinkel D, Straume TA, Gray J (1986) Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proc Natl Acad Sci USA 83:2934–2938
Liehr T (2010) Fluorescence in situ hybridization (FISH)—quality issues in molecular cytogenetics. In: Kristoffersson U, Schmidtke J, Cassiman J-J (eds) Quality issues in clinical genetic services. Springer Science+Business Media, New York, pp 315–320
van Stedum S, King W (2002) Basic FISH technique and troubleshooting. In: Fan Y-S (ed) Molecular cytogenetics: protocols and applications, vol 204. Humana Press, Totowa, pp 51–63
Chin S-F, Daigo Y, Huang H et al (2003) A simple and reliable pretreatment protocol facilitates fluorescent in situ hybridisation on tissue microarrays of paraffin wax embedded tumour samples. Mol Pathol 56:275–279
Dwight T, Thoppe SR, Foukakis T, Lui WO, Wallin G, Hoog A, Frisk T, Larsson C, Zedenius J (2003) Involvement of the PAX8/peroxisome proliferator-activated receptor gamma rearrangement in follicular thyroid tumors. J Clin Endocrinol Metab 88:4440–4445
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media New York
About this protocol
Cite this protocol
Sharifah, N.A.S.H., Zakaria, Z., Chia, W.K. (2013). FISH Analysis Using PPAR γ-Specific Probes for Detection of PAX8-PPAR γ Translocation in Follicular Thyroid Neoplasms. In: Badr, M., Youssef, J. (eds) Peroxisome Proliferator-Activated Receptors (PPARs). Methods in Molecular Biology, vol 952. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-155-4_13
Download citation
DOI: https://doi.org/10.1007/978-1-62703-155-4_13
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-154-7
Online ISBN: 978-1-62703-155-4
eBook Packages: Springer Protocols