Abstract
This chapter describes two basic complementary methods relevant to at least three major macronutrients in plants: NO −3 , SO 2−4 , and phosphate. The first method is the simultaneous determination of tissue content of the oxyanions, NO −3 , SO 2−4 , and phosphate by HPLC, and the second is the determination of tissue uptake (transport) capacity for these same oxyanions. NO −3 , phosphate, and SO 2−4 , as well as other anions including chloride, malate, and nitrite are extracted from milligram quantities of plant tissue and are separated and quantified in a single chromatographic (HPLC) run. Information on uptake (flux) of these same anions through the roots may be obtained using isotopically labeled elements, enabling transport capacity of roots and subsequent translocation to shoot tissues to be determined.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Tabataba MA, Bremner JM (1970) Simple turbidimetric method of determining total sulfur in plant materials. Agron J 62:805–810
Blake-Kalff MMA, Hawkesford MJ, Zhao FJ, McGrath SP (2000) Diagnosing sulfur deficiency in field-grown oilseed rape (Brassica napus L.) and wheat (Triticum aestivum L.). Plant Soil 225:95–107
Clarkson DT, Gojon A, Saker LR, Wiersema PK, Purves JV, Tillard P, Arnold GM, Paans AJM, Vaalburg W, Stulen I (1996) Nitrate and ammonium influxes in soybean (Glycine max) roots: direct comparison of N-13 and N-15 tracing. Plant Cell Environ 19:859–868
Smith FW, Hawkesford MJ, Ealing PM, Clarkson DT, Vanden Berg PJ, Belcher AR, Warrilow AGS (1997) Regulation of expression of a cDNA from barley roots encoding a high affinity sulphate transporter. Plant J 12:875–884
Blake-Kalff MMA, Harrison KR, Hawkesford MJ, Zhao FJ, McGrath SP (1998) Distribution of sulfur within oilseed rape leaves in response to sulfur deficiency during vegetative growth. Plant Physiol 118:1337–1344
Buchner P, Stuiver CEE, Westerman S, Wirtz M, Hell R, Hawkesford MJ, De Kok LJ (2004) Regulation of sulfate uptake and expression of sulfate transporter genes in Brassica oleracea as affected by atmospheric H2S and pedospheric sulfate nutrition. Plant Physiol 136:3396–3408
Brown DA, Clark LJ, Howarth JR, Parmar S, Hawkesford MJ (2006) Mechanical impedance and nutrient acquisition in rice. Plant Soil 280:65–76
Koralewska A, Buchner P, Stuiver CEE, Posthumus FS, Kopriva S, Hawkesford MJ, De Kok LJ (2009) Expression and activity of sulfate transporters and APS reductase in curly kale in response to sulfate deprivation and re-supply. J Plant Physiol 166:168–179
Clarkson DT, Diogo E, Amancio S (1999) Uptake and assimilation of sulphate by sulphur deficient Zea mays cells: the role of O-acetyl-L-serine in the interaction between nitrogen and sulphur assimilatory pathways. Plant Physiol Biochem 37:283–290
Lee RB (1982) Selectivity and kinetics of ion uptake by barley plants following nutrient deficiency. Ann Bot 50:429–449
Hawkesford MJ, Belcher AR (1991) Differential protein synthesis in response to sulfate and phosphate deprivation – identification of possible components of plasma-membrane transport-systems in cultured tomato roots. Planta 185:323–329
Acknowledgement
This research is funded by the Biotechnology and Biological Sciences Research Council (BBSRC) of the UK.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Hawkesford, M.J., Parmar, S., Buchner, P. (2013). Mineral Composition Analysis: Measuring Anion Uptake and Anion Concentrations in Plant Tissues. In: Maathuis, F. (eds) Plant Mineral Nutrients. Methods in Molecular Biology, vol 953. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-152-3_7
Download citation
DOI: https://doi.org/10.1007/978-1-62703-152-3_7
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-151-6
Online ISBN: 978-1-62703-152-3
eBook Packages: Springer Protocols