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Plant Cell Suspension Cultures

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Plant Mineral Nutrients

Part of the book series: Methods in Molecular Biology ((MIMB,volume 953))

Abstract

Plant cell suspension cultures are widely used in plant biology as a convenient tool for the investigation of a wide range of phenomena, bypassing the structural complexity of the plant organism in toto. The homogeneity of an in vitro cell population, the large availability of material, the high rate of cell growth and the good reproducibility of conditions make suspension-cultured cells suitable for the analysis of complex physiological processes at the cellular and molecular levels. Moreover, plant cell cultures provide a valuable platform for the production of high-value secondary metabolites and other substances of commercial interest. Here we describe how to initiate and maintain plant cell cultures starting from explants obtained from in vitro germinated seedlings. Isolation of protoplasts from plant cell suspension cultures and regeneration of plants via organogenesis and somatic embryogenesis are also presented.

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Acknowledgements

This work was supported by Programmi di Ricerca Scientifica di Rilevante Interesse Nazionale (2008WKPAWW to LN) and Ricerca Scientifica quota EX-60% (60A06-2248/11 to LN and 60A06-2448/11 to BB).

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Authors and Affiliations

  1. Department of Biology, University of Padova, Padova, Italy

    Roberto Moscatiello & Barbara Baldan

  2. Dipartimento di Biologia, Università di Padova, Padova, Italy

    Lorella Navazio

Authors
  1. Roberto Moscatiello
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  2. Barbara Baldan
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  3. Lorella Navazio
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Corresponding author

Correspondence to Lorella Navazio .

Editor information

Editors and Affiliations

  1. University of York, Biology Department Area 9, York, YO10 5DD, United Kingdom

    Frans J.M. Maathuis

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Moscatiello, R., Baldan, B., Navazio, L. (2013). Plant Cell Suspension Cultures. In: Maathuis, F. (eds) Plant Mineral Nutrients. Methods in Molecular Biology, vol 953. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-152-3_5

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  • DOI: https://doi.org/10.1007/978-1-62703-152-3_5

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-151-6

  • Online ISBN: 978-1-62703-152-3

  • eBook Packages: Springer Protocols

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