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Flux Measurements of Cations Using Radioactive Tracers

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Plant Mineral Nutrients

Part of the book series: Methods in Molecular Biology ((MIMB,volume 953))

Abstract

Standard procedures for the tracing of ion fluxes into roots of plants are described here, with emphasis on cations, especially potassium (K+). We focus in particular on the measurement of unidirectional influx by use of radiotracers and provide a brief introduction to compartmental analysis by tracer efflux (CATE).

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References

  1. de Hevesy G (1962) Adventures in radioisotope research: the collected papers of George Hevesy. Pergamon, New York

    Book  Google Scholar 

  2. Epstein E (1953) Mechanism of ion absorption by roots. Nature 171:83–84

    Article  PubMed  CAS  Google Scholar 

  3. Britto DT, Kronzucker HJ (2001) Can unidirectional influx be measured in higher plants? A mathematical approach using parameters from efflux analysis. New Phytol 150:37–47

    Article  CAS  Google Scholar 

  4. van Beusichem ML, Kirkby EA, Baas R (1988) Influence of nitrate and ammonium nutrition on the uptake, assimilation, and distribution of nutrients in Ricinus communis. Plant Physiol 86:914–921

    Article  PubMed  Google Scholar 

  5. Speer M, Brune A, Kaiser WM (1994) Replacement of nitrate by ammonium as the nitrogen source increases the salt sensitivity of pea plants. I. Ion concentrations in roots and leaves. Plant Cell Environ 17:1215–1221

    Article  Google Scholar 

  6. Britto DT, Kronzucker HJ (2005) Nitrogen acquisition, PEP carboxylase, and cellular pH homeostasis: new views on old paradigms. Plant Cell Environ 28:1396–1409

    Article  CAS  Google Scholar 

  7. Britto DT, Kronzucker HJ (2006) Futile cycling at the plasma membrane: a hallmark of low-affinity nutrient transport. Trends Plant Sci 11:529–534

    Article  PubMed  CAS  Google Scholar 

  8. Glass ADM (1978) Influence of incision and aging upon K+ influx on developing barley roots. Plant Physiol 61:481–483

    Article  PubMed  CAS  Google Scholar 

  9. Horie T, Costa A, Kim TH et al (2007) Rice OsHKT2;1 transporter mediates large Na+ influx component into K+-starved rice roots for growth. EMBO J 26:3003–3014

    Article  PubMed  CAS  Google Scholar 

  10. Britto DT, Kronzucker HJ (2008) Cellular mechanisms of potassium transport in plants. Physiol Plant 133:637–650

    Article  PubMed  CAS  Google Scholar 

  11. Brownridge JD (1984) The radial distribution of 137Cs and 40K in tree stems. J Plant Nutr 7:887–896

    Article  CAS  Google Scholar 

  12. Maier-Maercker U, Jahnke A (1980) Microautoradiography with 43K: a method for the reliable tracing of ion transport in stomata. Z Pflanzenphysiol 100:35–42

    CAS  Google Scholar 

  13. Kronzucker HJ, Siddiqi MY, Glass ADM (1995) Analysis of 13NH +4 efflux in spruce roots: a test case for phase identification in compartmental analysis. Plant Physiol 109:481–490

    PubMed  CAS  Google Scholar 

  14. Britto DT, Kronzucker HJ (2003) Trans-stimulation of 13NH +4 efflux provides evidence for the cytosolic origin of tracer in the compartmental analysis of barley roots. Funct Plant Biol 30:1233–1238

    Article  CAS  Google Scholar 

  15. Britto DT, Kronzucker HJ (2003) Cytosolic ion exchange dynamics: insights into the mechanisms of component ion fluxes and their measurement. Funct Plant Biol 30:355–363

    Article  CAS  Google Scholar 

  16. Malagoli P, Britto DT, Schulze LM et al (2008) Futile Na+ cycling at the root plasma membrane in rice (Oryza sativa L.) – kinetics, energetics, and relation to salinity tolerance. J Exp Bot 59:4109–4117

    Article  PubMed  CAS  Google Scholar 

  17. Coskun D, Britto DT, Kronzucker HJ (2010) Regulation and mechanism of potassium release from barley roots: an in planta 42K analysis. New Phytol 188:1028–1038

    Article  PubMed  CAS  Google Scholar 

  18. Szczerba MW, Britto DT, Kronzucker HJ (2006) Rapid, futile K+ cycling and pool-size dynamics define low-affinity potassium transport in barley. Plant Physiol 141:1494–1507

    Article  PubMed  CAS  Google Scholar 

  19. Epstein E (1966) Dual pattern of ion absorption by plant cells and by plants. Nature 212:1324–1327

    Article  CAS  Google Scholar 

  20. Britto DT, Kronzucker HJ (2009) Ussing’s conundrum and the search for transport mechanisms in plants. New Phytol 183:243–246

    Article  PubMed  CAS  Google Scholar 

  21. Lazof D, Cheeseman JM (1988) Sodium and potassium compartmentation and transport in the roots of intact lettuce plants. Plant Physiol 88:1279–1284

    Article  PubMed  CAS  Google Scholar 

  22. Yeo AR, Yeo ME, Flowers TJ (1987) The contribution of an apoplastic pathway to sodium uptake by rice roots in saline conditions. J Exp Bot 38:1141–1153

    Article  CAS  Google Scholar 

  23. Jacquez JA (1996) Compartmental analysis in biology and medicine, 3rd edn. U Michigan, Ann Arbor

    Google Scholar 

  24. Kronzucker HJ, Szczerba MW, Moazami-Goudarzi M et al (2006) The cytosolic Na+:K+ ratio does not explain salinity-induced growth impairment in barley: a dual-tracer study using 42K+ and 24Na+. Plant Cell Environ 29:2228–2237

    Article  PubMed  CAS  Google Scholar 

  25. Hajibagheri MA, Flowers TJ, Collins JC et al (1988) A comparison of the methods of X-ray microanalysis, compartmental analysis and longitudinal ion profiles to estimate cytoplasmic ion concentrations in two maize varieties. J Exp Bot 39:279–290

    Article  CAS  Google Scholar 

  26. Ashworth S, Rabiner EA, Gunn RN et al (2010) Evaluation of 11C-GSK189254 as a novel radioligand for the H3 receptor in humans using PET. J Nucl Med 51:1021–1029

    Article  PubMed  CAS  Google Scholar 

  27. Walker NA, Pitman MG (1976) Measurement of fluxes across membranes. In: Lüttge U, Pitman MG (eds) Encyclopedia of plant physiology, vol 2 Part A. Springer, Berlin

    Google Scholar 

  28. Siddiqi MY, Glass ADM, Ruth TJ (1991) Studies of the uptake of nitrate in barley. III. Compartmentation of NO −3 . J Exp Bot 42:1455–1463

    Article  CAS  Google Scholar 

  29. Cheeseman JM (1986) Compartmental efflux analysis – an evaluation of the technique and its limitations. Plant Physiol 80:51–64

    Article  Google Scholar 

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Correspondence to H. J. Kronzucker .

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Britto, D.T., Kronzucker, H.J. (2013). Flux Measurements of Cations Using Radioactive Tracers. In: Maathuis, F. (eds) Plant Mineral Nutrients. Methods in Molecular Biology, vol 953. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-152-3_10

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  • DOI: https://doi.org/10.1007/978-1-62703-152-3_10

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-151-6

  • Online ISBN: 978-1-62703-152-3

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