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Synthesis of Polyethylenimine-Based Nanocarriers for Systemic Tumor Targeting of Nucleic Acids

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Nanotechnology for Nucleic Acid Delivery

Part of the book series: Methods in Molecular Biology ((MIMB,volume 948))

Abstract

Nucleic acid-based therapies offer the option to treat tumors in a highly selective way, while toxicity towards healthy tissue can be avoided when proper delivery vehicles are used. We have recently developed carrier systems based on linear polyethylenimine, which after chemical coupling of proteinous or peptidic ligands can form nanosized polyplexes with plasmid DNA or RNA and deliver their payload into target cells by receptor-mediated endocytosis. This chapter describes the synthesis of linear PEI (LPEI) from a precursor polymer and the current coupling techniques and purification procedure for peptide conjugates with linear polyethylenimine. A protocol is also given for the formation and characterization of polyplexes formed with LPEI conjugate and plasmid DNA.

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Acknowledgments

This work was supported by the Center for Nanoscience (CeNS) and the German Research Foundation (SFB824) to M.O., and the Nanosystems Initiative Munich (NIM) to E.W.

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Authors and Affiliations

  1. Department of Pharmacy, Center for System Based Drug Research, Pharmaceutical Biotechnology, Ludwig-Maximilians-University, Munich, Germany

    Wolfgang Rödl

  2. Department of Pharmacy, Center for System based Drug Research, Pharmaceutical Biotechnology, Ludwig-Maximilians-University, Munich, Germany

    David Schaffert & Ernst Wagner

  3. Department of Molecular Biology, Aarhus University, Aarhus C, Denmark

    David Schaffert

  4. Center for NanoScience (CeNS), Ludwig-Maximilians-University, Munich, Germany

    Ernst Wagner & Manfred Ogris

  5. Center for System based Drug Research, Department of Pharmacy, Pharmaceutical Biotechnology, Ludwig-Maximilians-University, Munich, Germany

    Manfred Ogris

Authors
  1. Wolfgang Rödl
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  2. David Schaffert
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  3. Ernst Wagner
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  4. Manfred Ogris
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Corresponding author

Correspondence to Manfred Ogris .

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Editors and Affiliations

  1. , Department of Pharmacy, Ludwig-Maximilians-University Munich, Butenandstraße 5-13, Munich, 81377, Germany

    Manfred Ogris

  2. , Department of Pharmaceutical Sciences, Wayne State University, Mack Avenue 259, Detroit, 48201, USA

    David Oupicky

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Rödl, W., Schaffert, D., Wagner, E., Ogris, M. (2013). Synthesis of Polyethylenimine-Based Nanocarriers for Systemic Tumor Targeting of Nucleic Acids. In: Ogris, M., Oupicky, D. (eds) Nanotechnology for Nucleic Acid Delivery. Methods in Molecular Biology, vol 948. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-140-0_8

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  • DOI: https://doi.org/10.1007/978-1-62703-140-0_8

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-139-4

  • Online ISBN: 978-1-62703-140-0

  • eBook Packages: Springer Protocols

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