Protocol

Basic Cell Culture Protocols

Volume 946 of the series Methods in Molecular Biology pp 523-533

Date:

Formation of Embryoid Bodies from Human Pluripotent Stem Cells Using AggreWell™ Plates

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Abstract

Many human embryonic stem (hES) and induced pluripotent stem (hiPS) cell differentiation protocols begin with the formation of three-dimensional aggregates of cells called embryoid bodies (EBs). Traditional EB formation methods result in a heterogeneous population of EB sizes and shapes, which then undergo heterogeneous differentiation efficiencies. AggreWellTM400 and AggreWellTM800 use the spin-EB method to force the aggregation of a defined number of cells, thereby controlling EB size and generating a population of uniform EBs. Moreover, the dense array of microwells on the bottom surface of AggreWellTM400 provide for the rapid and simple production of thousands of EBs at a time.

Key words

Human embryonic stem cell Embryoid bodies Induced pluripotent stem cells