Protocol

Basic Cell Culture Protocols

Volume 946 of the series Methods in Molecular Biology pp 15-26

Date:

Eradication of Mycoplasma Contaminations

  • Cord C. UphoffAffiliated withDepartment of Human and Animal Cell Lines, DSMZ, German Collection of Microorganisms and Cell Cultures Email author 
  • , Hans G. DrexlerAffiliated withDepartment of Human and Animal Cell Lines, DSMZ, German Collection of Microorganisms and Cell Cultures and German Biological Resource Center

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Abstract

Mycoplasma contaminations have a multitude of effects on the cultured cell lines that may influence the results of experiments or pollute bioactive substances used in human medicine. The elimination of mycoplasma contaminations of cell cultures has become a practical alternative to discarding and reestablishing important or irreplaceable cell lines. Different quinolones, tetracyclines, and pleuromutilins shown to have strong antimycoplasma properties are employed for the decontamination. We provide detailed protocols to assure eradication of mycoplasma, to prevent formation of resistant mycoplasma strains, and to cure heavily contaminated and damaged cells. To date, we have not detected any consistent and permanent alterations to eukaryotic cells either during or after the treatment.

Key words

Antibiotic elimination Cell lines Mycoplasma