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Mouse Epidermal Keratinocyte Culture

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Book cover Epithelial Cell Culture Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 945))

Abstract

Cultured mouse epidermal keratinocytes provide a powerful model for studying epidermal proliferation, differentiation, pathogenesis, and oncogenic transformation. Primary mouse keratinocytes can be isolated from newborn mice, and their growth and differentiation can be manipulated by changing calcium concentrations in culture medium. Primary mouse keratinocytes proliferate in medium containing 0.05 mM calcium but cease to proliferate and start to terminally differentiate, when the calcium concentration in the culture medium is increased to 0.10 mM or greater.

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Acknowledgements

The author would like to thank Dr. Ulrike Lichti for her advice and helpful discussion. This work was supported by the intramural program of the NIH, Center for Cancer Research, National Cancer Institute.

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Authors and Affiliations

  1. Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA

    Luowei Li

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  1. Luowei Li
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Correspondence to Luowei Li .

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Editors and Affiliations

  1. Cystic Fibrosis/Pulmonary Research Ctr., University of North Carolina, Thurston Bowles Building 4011, Chapel Hill, 27599, North Carolina, USA

    Scott H. Randell

  2. Cystic Fibrosis/Pulmonary Research Ctr., The University of North Carolina, Thurston-Bowles Building 4011, Chapel Hill, 27599, North Carolina, USA

    M. Leslie Fulcher

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Li, L. (2012). Mouse Epidermal Keratinocyte Culture. In: Randell, S., Fulcher, M. (eds) Epithelial Cell Culture Protocols. Methods in Molecular Biology, vol 945. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-125-7_12

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  • DOI: https://doi.org/10.1007/978-1-62703-125-7_12

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-124-0

  • Online ISBN: 978-1-62703-125-7

  • eBook Packages: Springer Protocols

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