Abstract
In vitro transcription of RNA from DNA templates by T7 RNA polymerase allows for the generation of large quantities of RNA suitable for many downstream applications. The resulting RNA can be purified by a number of methodologies. Herein, we describe the native isolation of RNA molecules by FPLC purification using Superdex 75 or 200 gel filtration columns. This approach can be extended to purify biologically interesting RNA complexes such as RNA–protein complexes that have been generated from either synthetic or in vitro transcribed RNAs and recombinant proteins.
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Acknowledgments
The work was supported by a Natural Sciences and Engineering Research Council of Canada Discovery Grant and a Manitoba Health Research Council Establishment Grant.
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Booy, E.P., Meng, H., McKenna, S.A. (2013). Native RNA Purification by Gel Filtration Chromatography. In: Conn, G. (eds) Recombinant and In Vitro RNA Synthesis. Methods in Molecular Biology, vol 941. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-113-4_6
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DOI: https://doi.org/10.1007/978-1-62703-113-4_6
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Publisher Name: Humana Press, Totowa, NJ
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