Protocol

Phytoplasma

Volume 938 of the series Methods in Molecular Biology pp 159-171

Date:

Nested PCR and RFLP Analysis Based on the 16S rRNA Gene

  • Bojan DudukAffiliated withLaboratory of Applied Phytopathology, Institute of Pesticides and Environmental Protection Email author 
  • , Samanta PaltrinieriAffiliated withDiSTA, Plant Pathology, Alma Mater Studiorum—University of Bologna
  • , Ing-Ming LeeAffiliated withMolecular Plant Pathology Laboratory, USDA, ARS
  • , Assunta BertacciniAffiliated withDiSTA, Plant Pathology, Alma Mater Studiorum—University of Bologna

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Abstract

Current phytoplasma detection and identification methods are primarily based on nested polymerase chain reaction followed by restriction fragment length polymorphism analysis and gel electrophoresis. These methods can potentially detect and differentiate all phytoplasmas including those previously not described. The present protocol describes the application of this method for identification of phytoplasmas at 16S rRNA (16Sr) group and 16Sr subgroup levels.

Key words

Detection Identification Ribosomal group Ribosomal subgroup