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Vector-Free Methods for Manipulating miRNA Activity In Vitro and In Vivo

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MicroRNA Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 936))

Abstract

Vector-based methods for manipulating microRNA (miRNA) activity in vivo and in vitro face a number of disadvantages regarding biosafety, workload, and potential for therapeutic use in patients. Use of miRNA mimics and inhibitors provides an alternative for enhancing or inhibiting translational repression of target genes that could be easier applied to elucidate miRNA function. Here, we describe in detail application of miRNA mimics and inhibitors in vitro using adherent cell lines. In addition, we describe a method how they could be applied in vivo in a possible therapeutic context and provide a protocol for validation of this intervention using miRNA target protectors. We present these methods within a standard experimental design workflow that could be followed and we discuss the technical challenges that have to be taken into account.

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Author information

Authors and Affiliations

  1. Institute of Human Genetics, University of Goettingen, Goettingen, Germany

    Angeliki Pantazi

  2. Genome Sciences Center, BC Cancer Agency, Vancouver, BC, Canada

    Athanasios Zovoilis

Authors
  1. Angeliki Pantazi
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  2. Athanasios Zovoilis
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Corresponding author

Correspondence to Athanasios Zovoilis .

Editor information

Editors and Affiliations

  1. Keck School of Medicine, Dept. of Cell and Neurobiology, University of Southern California, San Pablo St. 1333, Los Angeles, 90033, California, USA

    Shao-Yao Ying

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Pantazi, A., Zovoilis, A. (2013). Vector-Free Methods for Manipulating miRNA Activity In Vitro and In Vivo. In: Ying, SY. (eds) MicroRNA Protocols. Methods in Molecular Biology, vol 936. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-083-0_18

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  • DOI: https://doi.org/10.1007/978-1-62703-083-0_18

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-082-3

  • Online ISBN: 978-1-62703-083-0

  • eBook Packages: Springer Protocols

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