Protocol

Protocols for Micropropagation of Selected Economically-Important Horticultural Plants

Volume 994 of the series Methods in Molecular Biology pp 45-62

Date:

Micropropagation Systems of Feijoa (Acca sellowiana (O. Berg) Burret)

  • Miguel Pedro GuerraAffiliated withLaboratory of Plant Developmental Physiology and Genetics, Graduate Program in Plant Genetic Resources, Federal University of Santa Catarina Email author 
  • , Gabriela Claudia Cangahuala-InocenteAffiliated withLaboratory of Plant Developmental Physiology and Genetics, Graduate Program in Plant Genetic Resources, Federal University of Santa Catarina
  • , Lirio Luiz Dal VescoAffiliated withLaboratory of Plant Developmental Physiology and Genetics, Graduate Program in Plant Genetic Resources, Federal University of Santa Catarina
  • , Rosete PescadorAffiliated withLaboratory of Plant Developmental Physiology and Genetics, Graduate Program in Plant Genetic Resources, Federal University of Santa Catarina
  • , Clarissa Alves CaprestanoAffiliated withLaboratory of Plant Developmental Physiology and Genetics, Graduate Program in Plant Genetic Resources, Federal University of Santa Catarina

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Abstract

Acca sellowiana (O. Berg) Burret sin. Feijoa sellowiana (Myrtaceae) is a semiwoody fruit species native to South Brazil, Uruguay, and Argentina; edible fruits are tasty. The naturally occurring populations in Santa Catarina State show high variability in fruit size, color, and other features. A breeding program launched in 1990 resulted in the release of four Brazilian commercial varieties. The conventional clonal propagation methods of this species, such as cutting and grafting, have shown low efficiency. Therefore, tissue culture techniques were developed for mass propagation. This chapter describes several protocols based on organogenesis and somatic embryogenesis. Additional techniques including synthetic seed technology and temporary immersion system are also described.

Key words

Artificial seed Feijoa sellowiana Mass propagation Myrtaceae Organogenesis Somatic embryogenesis Tissue culture Zygotic embryo