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In Vitro Propagation of Jojoba

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Protocols for Micropropagation of Selected Economically-Important Horticultural Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 994))

Abstract

Jojoba (Simmondsia chinensis (Link) Schn.) is a nontraditional crop in arid and semi-arid areas. Vegetative propagation can be achieved by layering, grafting, or rooting semi-hardwood cuttings, but the highest number of possible propagules is limited by the size of the plants and time of the year. Micropropagation is highly recommended strategy for obtaining jojoba elite clones. For culture initiation, single-node explants are cultivated on Murashige and Skoog medium (MS) supplemented with Gamborg’s vitamins (B5), 11.1 μM BA (N6-benzyl-adenine), 0.5 μM IBA (indole-3-butyric acid), and 1.4 μM GA3 (gibberellic acid). Internodal and apical cuttings proliferate on MS medium containing B5 vitamins and 4.4 μM BA. Rooting is achieved on MS medium (half strength mineral salt) amended with B5 vitamins and 14.7 μM IBA during 7 days and transferred to develop in auxin-free rooting medium. Plantlets are acclimatized using a graduated humidity regime on soil: peat: perlite (5:1:1) substrate. This micropagation protocol produces large numbers of uniform plants from selected genotypes of jojoba.

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Author information

Authors and Affiliations

  1. Plant Tissue Culture Laboratory (CULTEV), Department of Basic Sciences, National University of Luján, Buenos Aires, Argentina

    Berta E. Llorente

  2. Department of Basic Sciences, Plant Tissue Culture Laboratory (CULTEV), National University of Luján, Buenos Aires, Argentina

    Nancy M. Apóstolo

Authors
  1. Berta E. Llorente
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  2. Nancy M. Apóstolo
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Corresponding author

Correspondence to Berta E. Llorente .

Editor information

Editors and Affiliations

  1. , IVALSA/Istituto per la Valorizzazione, National Research Council (CNR), Polo Scientifico, via Madonna del Piano n. 10, Sesto Fiorentino, Florence, 50019, Italy

    Maurizio Lambardi

  2. , Istituto per la Valorizzazione del Legno, National Research Council (CNR), n/a, Florence, n/a, Italy

    Elif Aylin Ozudogru

  3. , Department of Agricultural, University of Helsinki, 5 Latokartanonkaari Room C 130, Helsinki, 00014, Finland

    Shri Mohan Jain

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Llorente, B.E., Apóstolo, N.M. (2012). In Vitro Propagation of Jojoba. In: Lambardi, M., Ozudogru, E., Jain, S. (eds) Protocols for Micropropagation of Selected Economically-Important Horticultural Plants. Methods in Molecular Biology, vol 994. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-074-8_2

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  • DOI: https://doi.org/10.1007/978-1-62703-074-8_2

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-073-1

  • Online ISBN: 978-1-62703-074-8

  • eBook Packages: Springer Protocols

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