Protocol

Protocols for Micropropagation of Selected Economically-Important Horticultural Plants

Volume 994 of the series Methods in Molecular Biology pp 179-186

Date:

Micropropagation of Chokeberry by In Vitro Axillary Shoot Proliferation

  • Wojciech LitwińczukAffiliated withFaculty of Biology and Agriculture, Department of Plant Physiology and Biotechnology, University of Rzeszów Email author 

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Abstract

The black chokeberry—aronia (Aronia melanocarpa Elliot) is a shrub native to North America although nowadays well known in Eastern Europe. The fruits are regarded as the richest source of antioxidant phytonutrients among fruit crops and vegetables. Chokeberries can be easily propagated by seeds but this method is not recommended. Micropropagation is far more efficient than other conventional cloning methods like layering or softwood cuttings. Aronia clones are propagated in vitro through four- or three-stage method based on subculturing of shoot explants. The double diluted MS or full strength MS medium with elevated 50% Ca2+ and Mg2+ content are used in the initiation and proliferation chokeberry in vitro cultures, respectively. They are supplemented with 0.5–1.0 mg LBA, and 0.05 mg LIBA. The double-phase medium is recommended in the last passage before shoot rooting. The regenerated shoots could be rooted both in vitro on double diluted MS with 0.05 mg L−1 IBA or in vivo in peat and perlite substrate and subsequently grown in the greenhouse.

Key words

Aronia Axillary shoots Nodal explants Small fruits Tissue culture