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Methods of Sperm Vitality Assessment

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Spermatogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 927))

Abstract

Sperm vitality is a reflection of the proportion of live, membrane-intact spermatozoa determined by either dye exclusion or osmoregulatory capacity under hypo-osmotic conditions. In this chapter we address the two most common methods of sperm vitality assessment: eosin–nigrosin staining and the hypo-osmotic swelling test, both utilized in clinical Andrology laboratories.

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References

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Author information

Authors and Affiliations

  1. CReATe Fertility Center, Toronto, ON, Canada

    Sergey I. Moskovtsev

  2. Department of Obstetrics & Gynaecology, University of Toronto, Toronto, ON, Canada

    Sergey I. Moskovtsev

  3. Division of Reproductive Endocrinology and Infertility, Department of Obstetrics & Gynaecology, CReATe Fertility Center, Sunnybrook Health Sciences Centre and Women’s College Hospital, University of Toronto, Toronto, ON, Canada

    Clifford L. Librach

Authors
  1. Sergey I. Moskovtsev
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  2. Clifford L. Librach
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Corresponding author

Correspondence to Sergey I. Moskovtsev .

Editor information

Editors and Affiliations

  1. Andrology and IVF Laboratories, Department of Surgery (Urology), University of Utah School of Medicine, Arapeen Dr. 675, Salt Lake City, 84108, Utah, USA

    Douglas T. Carrell

  2. , Andrology and IVF Laboratories, University of Utah School of Medicine, Arapeen Way 675, Salt Lake City, 84108, Utah, USA

    Kenneth I. Aston

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© 2013 Springer Science+Business Media, LLC

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Moskovtsev, S.I., Librach, C.L. (2013). Methods of Sperm Vitality Assessment. In: Carrell, D., Aston, K. (eds) Spermatogenesis. Methods in Molecular Biology, vol 927. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-038-0_2

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  • DOI: https://doi.org/10.1007/978-1-62703-038-0_2

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-037-3

  • Online ISBN: 978-1-62703-038-0

  • eBook Packages: Springer Protocols

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