Skip to main content

Methods for Sperm Concentration Determination

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 927))

Abstract

Proper assessment of the number of spermatozoa is essential not only as an initial step in every clinical infertility investigation [Björndahl et al (2010) A practical guide to basic laboratory andrology, 1st edn. Cambridge University Press, Cambridge] but also when attempting to establish the total sperm production in the testis [Amann (Hum Reprod 25:22–28, 2010); Amann (J Androl 30:626–641, 2009); Amann and Chapman (J Androl 30:642–649, 2009)]. Reliable methods combined with an understanding of the specific physiology involved as well as the main sources of errors related to the assessment of sperm concentration are critical for ensuring accurate concentration determination [Björndahl et al (2010) A practical guide to basic laboratory andrology, 1st edn. Cambridge University Press, Cambridge; World Health Organization (2010) WHO laboratory manual for the examination and processing of human semen. WHO, Geneva]. This chapter therefore focuses on these three aspects.

This is a preview of subscription content, log in via an institution.

Buying options

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Björndahl L et al (2010) A practical guide to basic laboratory andrology, 1st edn. Cambridge University Press, Cambridge

    Google Scholar 

  2. Amann RP (2010) Evaluating testis function non-invasively: how epidemiologist–andrologist teams might better approach this task. Hum Reprod 25:22–28

    Article  PubMed  CAS  Google Scholar 

  3. Amann RP (2009) Considerations in evaluating human spermatogenesis on the basis of total sperm per ejaculate. J Androl 30:626–641

    Article  PubMed  Google Scholar 

  4. Amann RP, Chapman PL (2009) Total sperm per ejaculate of men: obtaining a meaningful value or a mean value with appropriate precision. J Androl 30:642–649

    Article  PubMed  Google Scholar 

  5. World Health Organization (2010) WHO laboratory manual for the examination and processing of human semen. WHO, Geneva

    Google Scholar 

  6. Cooper TG et al (2010) World Health Organization reference values for human semen characteristics. Hum Reprod Update 16:231–245

    Article  PubMed  Google Scholar 

  7. Björndahl L (2011) What is normal semen quality? On the use and abuse of reference limits for the interpretation of semen analysis results. Hum Fertil (Camb) 14(3):179–186

    Article  Google Scholar 

  8. Barratt CL, Bjorndahl L, Menkveld R, Mortimer D (2011) ESHRE special interest group for andrology basic semen analysis course: a continued focus on accuracy, quality, efficiency and clinical relevance. Human reproduction (Oxford, England) 26:3207–3212

    Google Scholar 

  9. Kirkman-Brown J, Björndahl L (2009) Evaluation of a disposable plastic Neubauer counting chamber for semen analysis. Fertil Steril 91:627–631

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Lars Björndahl .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2013 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Björndahl, L. (2013). Methods for Sperm Concentration Determination. In: Carrell, D., Aston, K. (eds) Spermatogenesis. Methods in Molecular Biology, vol 927. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-038-0_1

Download citation

  • DOI: https://doi.org/10.1007/978-1-62703-038-0_1

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-037-3

  • Online ISBN: 978-1-62703-038-0

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics