Abstract
Neurotransmitter amino acids like Alanine (Ala), Glutamic acid (Glu), Aspartic acid (Asp), Serine (Ser), Taurine (Tau), and Glycine (Gly) are widely distributed in biological fluids and tissues and they have important physiological functions, so that the evaluation of their levels in organisms plays an essential role in physiology. We here report a new capillary electrophoresis (CE) method with laser-induced fluorescence detection by which analytes are resolved and detected in less than 12 min both in human plasma and in other samples types, such as red blood cells, urine, cultured cells, cerebrospinal fluid, saliva, and vitreous humor, thus avoiding the typical limitations of the other methods normally suitable only in one or two matrix types.
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Acknowledgments
Angelo Zinellu was supported by Regione Autonoma della Sardegna “Ricerca cofinanziata PROGRAMMA OPERATIVO FSE SARDEGNA 2007–2013 -L.R.7/2007—Promozione della ricerca scientifica e dell’innovazione tecnologica in Sardegna.” The manuscript language revision by Mrs Maria Antonietta Meloni is greatly appreciated.
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Zinellu, A., Sotgia, S., Deiana, L., Carru, C. (2013). Analysis of Neurotransmitter Amino Acids by CE-LIF Detection in Biological Fluids. In: Phillips, T., Kalish, H. (eds) Clinical Applications of Capillary Electrophoresis. Methods in Molecular Biology, vol 919. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-029-8_4
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DOI: https://doi.org/10.1007/978-1-62703-029-8_4
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