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Malaria pp 99–125Cite as

Transfection of Rodent Malaria Parasites

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 923))

Abstract

Gene manipulation is an invaluable tool to investigate and understand the biology of an organism. Although this technology has been applied to both the human and rodent malarial parasites (RMP), Plasmodium berghei in particular offers a more robust system due to a higher and more efficient transformation rate. Here, we describe a comprehensive transfection and selection protocol using P. berghei including a variant negative selection protocol administering 5-fluorocytosine to the animals in drinking water. Additionally, we discuss and assess the latest advances in gene manipulation technologies developed in RMP to gain a better understanding of Plasmodium biology.

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Author information

Authors and Affiliations

  1. Division of Infection and Immunity Glasgow Biomedical Research Centre, Faculty of Biomedical Life Sciences and Wellcome Trust Center for Molecular Parasitology, University of Glasgow, Glasgow, UK

    Nisha Philip & Rachael Orr

  2. Division of Infection and Immunity Faculty of Biomedical Life Sciences and Wellcome Trust, Center for Molecular Parasitology Glasgow Biomedical Research Centre University of Glasgow, Glasgow, UK

    Andrew P. Waters

Authors
  1. Nisha Philip
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  2. Rachael Orr
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  3. Andrew P. Waters
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Corresponding author

Correspondence to Andrew P. Waters .

Editor information

Editors and Affiliations

  1. , Malaria Biology and Genetics Unit, Institut Pasteur, Rue du Dr Roux 28, Paris, 75724, France

    Robert Ménard

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Philip, N., Orr, R., Waters, A.P. (2012). Transfection of Rodent Malaria Parasites. In: Ménard, R. (eds) Malaria. Methods in Molecular Biology, vol 923. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-026-7_7

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  • DOI: https://doi.org/10.1007/978-1-62703-026-7_7

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-025-0

  • Online ISBN: 978-1-62703-026-7

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