Abstract
The isolation and culture of both embryonic and extraembryonic stem cells provide an enormous opportunity to study the molecular processes that establish and maintain lineage-specific, monoallelic patterns of gene expression. This chapter describes the isolation an culture of trophectoderm stem cells from mouse blastocyst stage embryos. Using this powerful in vitro system, scientists can now begin to tease apart the epigenetic processes that result in placental patterns of imprinted gene expression and begin to better understand the role these genes play in development and disease.
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Acknowledgement
This work was supported by the NIH grant AA020129-02.
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Golding, M.C. (2012). Generation of Trophoblast Stem Cells. In: Engel, N. (eds) Genomic Imprinting. Methods in Molecular Biology, vol 925. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-011-3_3
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DOI: https://doi.org/10.1007/978-1-62703-011-3_3
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-62703-011-3
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