Abstract
A spectacular advantage of Xenopus tropicalis is the ease with which diploid embryos can be generated year round. By the simple administration of human chorionic gonadotropin, an investigator can generate many hundreds of synchronized embryos by in vitro fertilization or thousands of embryos from a mating pair. The ability to induce ovulations when desired facilitates many different experiments such as experimental embryology, molecular manipulation of gene products, and genetics.
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References
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Acknowledgements
Many thanks to the Grainger lab for their protocol and John Gerhart for help with X. tropicalis IVF at the very beginning. Also many thanks to the students and instructors of the CSH Xenopus course which inspired testing many different IVF protocols.
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© 2012 Springer Science+Business Media, LLC
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del Viso, F., Khokha, M. (2012). Generating Diploid Embryos from Xenopus tropicalis . In: HOPPLER, S., Vize, P. (eds) Xenopus Protocols. Methods in Molecular Biology, vol 917. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-992-1_3
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DOI: https://doi.org/10.1007/978-1-61779-992-1_3
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-61779-992-1
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