Plant Salt Tolerance

Volume 913 of the series Methods in Molecular Biology pp 191-200


In vivo Imaging of Nitric Oxide and Reactive Oxygen Species Using Laser Scanning Confocal Microscopy

  • Yan-Jie XieAffiliated withCollege of Life Sciences, Nanjing Agricultural University
  • , Wen-Biao ShenAffiliated withCollege of Life Sciences, Nanjing Agricultural University Email author 

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Both nitric oxide (NO) and reactive oxygen species (ROS) are versatile molecules that mediate a variety of cellular responses in plants. In this chapter, methods for imaging NO and ROS using laser scanning confocal microscopy (LSCM) are presented. Arabidopsis roots, dyed with DAF-FM or H2DCF, are observed using the Leica TCS-SP2 LSCM. NO or ROS production are imaged and their kinetic changes monitored with the laser excitation and emission wavelengths at 488 nm and between 500 and 530 nm, respectively. In addition, Leica software is employed to visualize and calculate the fluorescence intensity data.

Key words

Nitric oxide Reactive oxygen species Laser scanning confocal microscopy