Abstract
Although the mouse is widely used as an animal model for the in vitro culture of human preimplantation embryos, arguments can be made for the validity of many domestic species as more relevant, applicable models of early human development. Here, we describe the advantages and disadvantages of domestic species as animal models to develop in vitro culture methods and conditions that successfully support human embryo development. In vitro embryo culture procedures are described and compared between species for the bovine, ovine, caprine, and porcine species.
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References
Bavister BD (2002) How animal embryo research led to the first documented human IVF. RBM Online 4(1):24–29
Wall RJ, Shani M (2008) Are animal models as good as we think? Theriogenology 69:2–9
van der Worp HB, Howells DW, Sena ES, Porritt MJ, Rewell S, O’Collins V, CMacleod MR (2010) Can animal models of disease reliably inform human studies? PLoS Med 7:e1000245
Lonergan P, Fair T (2008) In vitro-produced bovine embryos: dealing with the warts. Theriogenology 69:17–22
Keefer CL (2008) Lessons learned from nuclear transfer (cloning). Theriogenology 69:48–54
Prather RS, Shen M, Dai Y (2008) Genetically modified pigs for medicine and agriculture. Biotech Genet Eng Rev 25:245–266
Menezo YJR, Herubel F (2002) Mouse and bovine models for human IVF. RBM Online 4:170–175
Betteridge KJ, Rieger D (1993) Embryo transfer and related techniques in domestic animals, and their implications for human medicine. Hum Reprod 8:147–167
Carnevale EM (2008) The mare model for follicular maturation and reproductive aging in the woman. Theriogenology 69:23–30
Biggers JD, Whittingham DG, Donahue RP (1967) The pattern of energy metabolism in the mouse oocyte and zygote. PNAS 58:560–567
Leese HJ, Barton AM (1984) Pyruvate and glucose uptake by mouse ova and preimplantation embryos. J Reprod Fertil 72:9–13
Rieger D, Loskutoff NM, Betteridge KJ (1992) Developmentally related changes in the uptake and metabolism of glucose, glutamine and pyruvate by cattle embryos produced in vitro. Reprod Fertil Dev 4:547–557
Rieger D, Loskutoff NM, Betteridge KJ (1992) Developmentally related changes in the metabolism of glucose and glutamine by cattle embryos produced and co-cultured in vitro. J Reprod Fertil 95:585–595
Kim JH, Funahashi H, Niwa K, Okuda K (1993) Glucose requirement at different developmental stages of in vitro fertilized bovine embryos cultured in semi-defined medium. Theriogenology 39:875–886
Gardner DK, Lane M, Batt P (1993) Uptake and metabolism of pyruvate and glucose by individual sheep preattachment embryos developed in vivo. Mol Reprod Dev 36:313–319
Hardy K, Hooper MA, Handyside AH, Rutherford AJ, Winston RM, Leese HJ (1989) Non-invasive measurement of glucose and pyruvate uptake by individual human oocytes and preimplantation embryos. Hum Reprod 4:188–191
Conaghan J, Handyside AH, Winston RM, Leese HJ (1993) Effects of pyruvate and glucose on the development of human preimplantation embryos in vitro. J Reprod Fertil 99:87–95
Bavister BD (1995) Culture of preimplantation embryos: facts and artifacts. Hum Reprod Update 1:91–148
Leese HJ, Conaghan J, Martin KL, Hardy K (1993) Early human embryo metabolism. Bioessays 15:259–264
Swain JE, Bormann CL, Clark SG, Walters EM, Wheeler MB, Krisher RL (2002) Use of energy substrates by various stage preimplantation pig embryos produced in vivo and in vitro. Reproduction 123:253–260
Gandhi AP, Lane M, Gardner DK, Krisher RL (2001) Substrate utilization in porcine embryos cultured in NCSU23 and G1.2/G2.2 sequential culture media. Mol Reprod Dev 58:269–275
Harvey AJ, Kind KL, Thompson JG (2002) REDOX regulation of early embryo development. Reproduction 123:479–486
Matorras R, Ruiz JI, Mendoza R, Ruiz N, Sanjurjo P, Rodriguez-Escudero FJ (1998) Fatty acid composition of fertilization-failed human oocytes. Hum Reprod 13:2227–2230
McEvoy TG, Coull GD, Broadbent PJ, Hutchinson JS, Speake BK (2000) Fatty acid composition of lipids in immature cattle, pig and sheep oocytes with intact zona pellucida. J Reprod Fertil 118:163–170
Sturmey RG, Reis A, Leese HJ, McEvoy TG (2009) Role of fatty acids in energy provision during oocyte maturation and early embryo development. Reprod Dom Anim 44(3):50–58
Dunning KR, Cashman K, Russell DL, Thompson JG, Norman RJ, Robker RL (2010) Beta-oxidation is essential for mouse oocyte developmental competence and early embryo development. Biol Reprod 83:909–918
Haggarty P, Wood M, Ferguson E, Hoad G, Srikantharajah A, Milne E, Hamilton M, Bhattacharya S (2006) Fatty acid metabolism in human preimplantation embryos. Hum Reprod 21:766–773
Gandhi AP, Lane M, Gardner DK, Krisher RL (2000) A single medium supports development of bovine embryos throughout maturation, fertilization and culture. Hum Reprod 15:395–401
Tervit HR, Whittingham DG, Rowson LEA (1972) Successful culture in vitro of sheep and cattle ova. J Reprod Fertil 30:493–497
Petters RM, Wells KD (1993) Culture of pig embryos. J Reprod Fertil 48:61–73
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Krisher, R.L. (2012). Utility of Animal Models for Human Embryo Culture Development: Domestic Species. In: Smith, G., Swain, J., Pool, T. (eds) Embryo Culture. Methods in Molecular Biology, vol 912. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-971-6_3
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DOI: https://doi.org/10.1007/978-1-61779-971-6_3
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-61779-971-6
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