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Embryo Culture pp 325–331Cite as

Culture Systems: Mineral Oil Overlay

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 912))

Abstract

Mineral oil overlay microdrop is commonly used during in vitro fertilization (IVF) procedures. Though mineral oil appears homogeneous, it is an undefined product that can vary in quality. Here, we describe the history, chemistry, processing, and optimal use of mineral oil for IVF and embryo culture.

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Author information

Authors and Affiliations

  1. Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Mayo Clinic, Rochester, MN, USA

    Dean E. Morbeck & Phoebe H. Leonard

Authors
  1. Dean E. Morbeck
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  2. Phoebe H. Leonard
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Corresponding author

Correspondence to Dean E. Morbeck .

Editor information

Editors and Affiliations

  1. , OB/GYN, University of Michigan, E. Catherine Street 1137, Ann Arbor, 48109-5622, Michigan, USA

    Gary D. Smith

  2. , Center for Reproductive Medicine, University of Michigan, Market Street 475, Ann Arbor, 48108, Michigan, USA

    Jason E. Swain

  3. Fertility Center of San Antonio, Medical Drive 4499, San Antonio, 78229, Texas, USA

    Thomas B. Pool

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© 2012 Springer Science+Business Media New York

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Morbeck, D.E., Leonard, P.H. (2012). Culture Systems: Mineral Oil Overlay. In: Smith, G., Swain, J., Pool, T. (eds) Embryo Culture. Methods in Molecular Biology, vol 912. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-971-6_18

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  • DOI: https://doi.org/10.1007/978-1-61779-971-6_18

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-970-9

  • Online ISBN: 978-1-61779-971-6

  • eBook Packages: Springer Protocols

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