Abstract
The detergent-resistant membrane skeletons play a critical role in cell shaping and signaling. The focus of the methods described in this chapter is first on the preparation of membrane skeletons from liver by multistep sucrose density gradient centrifugation, and then on the analysis of the protein components of membrane skeletons using proteomics techniques. Two proteomic analysis strategies are described. In the first strategy, membrane skeleton proteins are separated by two-dimensional gel electrophoresis and identified by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry. In the other strategy, proteins are separated by SDS-PAGE and identified by liquid chromatography-electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry. The methods facilitate the understanding of the structure of membrane skeletons.
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Acknowledgements
This work was supported by grants from National Key Basic Research Program of China (No. 2010CB912203 and 2010CB915504) and National Natural Science Foundation of China (No. 90919023 and 30970652).
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He, J., Liu, Y., Wang, Q., Ji, J. (2012). Purification and Proteomic Analysis of Liver Membrane Skeletons. In: Josic, D., Hixson, D. (eds) Liver Proteomics. Methods in Molecular Biology, vol 909. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-959-4_7
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DOI: https://doi.org/10.1007/978-1-61779-959-4_7
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