Abstract
Oleaginous yeast Yarrowia lipolytica is capable of accumulating large amount of lipids. There is a growing interest to engineer this organism to produce lipid-derived compounds for a variety of applications. In addition, biosynthesis of value-added products such as carotenoid and its derivatives have been explored. In this chapter, we describe methods to integrate genes involved in lycopene biosynthesis in Yarrowia. Each bacterial gene involved in lycopene biosynthesis, crtE, crtB, and crtI, will be assembled with yeast promoters and terminators and subsequently transformed into Yarrowia through random integration. The engineered strain can produce lycopene under lipid accumulation conditions.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Bankar A, Kumar A, Zinjarde S (2009) Environmental and industrial applications of Yarrowia lipolytica. Appl Microbiol Biotechnol 84:847–865
Zhu Q, Xue Z, Yadav N, Damude H, Pollak DW, Rupert R, Seip J, Hollerback D, Macool D, Zhang H, Bledsoe S, Short D, Tyreus B, Kinney A, Picataggio S (2010) Metabolic engineering of an oleaginous yeast for the production of omega-3 fatty acids. In: Cohen Z, Ratledge C (eds) Single cell oil, 2nd edn. ACOS Press, Urbana, pp 51–73
Sabirova J, Haddouche R, Van Bogaert I, Mulaa F, Verstraete W, Timmis K, Schmidt-Dannert C, Nicaud J, Soetaert W (2010) The “LipoYeasts” project: using the oleaginous yeast Yarrowia lipolytica in combination with specific bacterial genes for the bioconversion of lipids, fats and oils into high-value products. Microb Biotechnol 4:47–54
Bhosale P, Bernstein P (2005) Microbial xanthophylls. Appl Microbiol Biotechnol 68:445–455
Misawa N, Shimada H (1997) Metabolic engineering for the production of carotenoids in non-carotenogenic bacteria and yeasts. J Biotechnol 59:169–181
Mauersberger S, Wang H, Gaillardin C, Barth G, Nicaud J (2001) Insertional mutagenesis in the n-alkane-assimilating yeast Yarrowia lipolytica: generation of tagged mutations in genes involved in hydrophobic substrate utilization. J Bacteriol 183:5102–5109
Ye R, Stead K, Yao H, He H (2006) Mutational and functional analysis of the beta-carotene ketolase involved in the production of canthaxanthin and astaxanthin. Appl Environ Microbiol 72:5829–5837
O’Fallon JV, Busboom JR, Nelson ML, Gaskins CT (2007) A direct method for fatty acid methyl ester synthesis: application to wet meat tissues, oils, and feedstuffs. J Anim Sci 85: 1511–1521
Yu X, Zheng Y, Dorgan KM, Chen S (2011) Oil production by oleaginous yeasts using the hydrolysate from pretreatment of wheat straw with dilute sulfuric acid. Bioresour Technol 102(10): 6134–6140
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media New York
About this protocol
Cite this protocol
Ye, R.W., Sharpe, P.L., Zhu, Q. (2012). Bioengineering of Oleaginous Yeast Yarrowia lipolytica for Lycopene Production. In: Barredo, JL. (eds) Microbial Carotenoids From Fungi. Methods in Molecular Biology, vol 898. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-918-1_9
Download citation
DOI: https://doi.org/10.1007/978-1-61779-918-1_9
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-917-4
Online ISBN: 978-1-61779-918-1
eBook Packages: Springer Protocols